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细胞名称

U-937人组织细胞淋巴瘤细胞

描述

U-937细胞系由Sundstrom和Nilsson在1974年建立，取材于患组织细胞淋巴瘤病人的胸水。 研究表明：U-937细胞能被人混合淋巴细胞培养上清，佛波脂、维生素D3、γ干扰素、肿瘤坏死因子和维甲酸诱导终末单核细胞分化。 该细胞免疫球蛋白产物和EB病毒表达为阴性。 该细胞表达Fas抗原且对TNF和抗-Fas抗体敏感。

动物种别

人

性别

男

组织来源

组织细胞淋巴瘤

形态

单核细胞

培养基和添加剂

RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

Sundstrom C and Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer 17:565-577, 1976 PubMed: 76189427 Ralph P , et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Gene expression during normal and malignant differentiation. 143: London: Academic Press; 1985, pp. 57-72. International symposium on new trends in human immunology and cancer immunotherapy. Paris: Doin Editeurs; 1980, pp. 271-292. Koren HS , et al. In vitro activation of a human macrophage-like cell line. Nature 279: 328-331, 1979. PubMed: 450085 Gidlund M , et al. Natural killer cells kill tumour cells at a given stage of differentiation. Nature 292: 848-850, 1981. PubMed: 7266653 Olsson I , et al. Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1 alpha,25-dihydroxycholecalciferol. Cancer Res. 43: 5862-5867, 1983. PubMed:6315218 Morimoto H , et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. PubMed: 7684321 Giovannangeli C , et al. Accessibility of nuclear DNA to triplex-forming oligonucleotides: The integrated HIV-1 provirus as a target. Proc. Natl. Acad. Sci. USA 94: 79-84, 1997. PubMed: 8990164 Brigino E , et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366 Reid YA , et al. Cell Line Cross-contamination of U-937. J. Leukocyte Biol. 57: 804, 1995. PubMed: 7759961 Sundstrom C , Nilsson K . Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer 17: 565-577, 1976. PubMed: 178611 Hay, R. J., Caputo, J. L, and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227. 1988. Fleming, D.O., Richardson, J. H., TuIis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.