当前位置：> > >

大鼠肝细胞

细胞名称	BRL 3A大鼠肝细胞
描述	这个细胞株的无血清条件培养基是MSA的一个来源。 MSA多肽家族能够部分满足鸡胚成纤维细胞对血清的需求。
动物种别	大鼠
性别	***
组织来源	肝
形态	***
培养基和添加剂	DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制	A。仅供研究之用。
REFERENCE	Nissley SP , et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146 Coon HG , Weiss MC . A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097 Dulak NC , Temin HM . A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141 Dulak NC , Shing YW . Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209 Schalch DS , et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052