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细胞名称 |
EL4小鼠淋巴瘤细胞 |
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描述 |
EL4是从用9,10-二甲基-1,2-苯并蒽在C57BL小鼠中诱导的淋巴瘤中建立的。 能抗0.1 mM 氢化可的松，对20 mcg/ml PHA敏感。 还有一个亚株(EL4.IL-2, ATCC TIB-181)可以生成高水平的IL-2。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 |
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动物种别 |
小鼠 |
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性别 |
*** |
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组织来源 |
T 淋巴细胞；淋巴瘤 |
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形态 |
淋巴母细胞 |
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培养基和添加剂 |
DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，90%；马血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 |
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供应限制
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A。仅供研究之用。 |
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REFERENCE |
Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 |
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中文名称
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小鼠淋巴瘤细胞 |
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