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细胞名称

HL-60人原髓细胞白血病细胞

描述

HL-60是一株早幼粒细胞。 外周血白细胞来自一位患有急性粒-单核细胞白血病的36岁白人女性。 HL-60 自发分化，丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸可以促进分化。 细胞表现出吞噬活性，并对趋化刺激有响应。致癌基因myc表达阳性。

动物种别

人

性别

女

组织来源

外周血；早幼粒细胞；急性粒－单核细胞白血病

形态

原粒细胞

培养基和添加剂

IMDM培养基(GIBCO,货号12200036)，80%；优质胎牛血清，20%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

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